Improved methods for isolation and enumeration of Malassezia furfur from human skin.
نویسندگان
چکیده
A medium for the isolation and enumeration of Malassezia furfur is described. Incubation at 34 degrees C yielded geometric mean counts (in CFU per square centimeter) of 2.6 X 10(3) on the forehead, 8.5 X 10(2) on the cheek, and 9.6 X 10(3) on the back. These counts compared favorably with microscopic counts and greatly exceeded those obtained with previously described media.
منابع مشابه
Natural Oils Enhance IL-10 and IFN-γ Production by Human PBMCs Cultured with Malassezia furfur
Background: Malassezia furfur is alipophilic yeast that causes skin disease. Objective: To evaluate the level of IL-10, IFN-γ and IL-12P70 in co-incubation of M. furfur grown on different forms of natural oils with PBMCs of healthy individuals. Methods: PBMCs were obtained from blood samples of normal volunteers. M. furfur was cultured in different culture media containing almond oil, fish oil,...
متن کاملتعیین گونه مالاسزیاهای جدا شده از بیماران مبتلا به پیتیر یازیس ورسیکالر و درماتیت سبوروئیک، با روش PCR-RFLP
Background and Purpose: Lipophilic yeast of the genus Malassezia are members of normal human cutaneous micro flora which are also associated with several skin diseases. It is strongly suspected that Malassezia species are responsible for pityriasis versicolor (PV), and seborrhoeic dermatitis (SD). Considering various sensitivities among Malassezia species to antifungal, accurate species identif...
متن کاملRapid Identification of Malassezia furfur from other Malassezia Species: A Major Causative Agent of Pityriasis Versicolor
The present study is designed to evaluate the application of a simple method for rapid identification of Malassezia furfur among other Malassezia spp. based on production of a brownish-red pigment and its diffusion into the medium containing L-tryptophan as a sigle source of nitrogen. 91 strains of Malassezia (20% M. furfur, 2% M. sympodialis, 5% M. obtosa and 73% M. globosa) isolated from ski...
متن کاملDistribution of Malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods
BACKGROUND The Malassezia yeasts which belong to the physiological microflora of human skin have also been implicated in several dermatological disorders, including pityriasis versicolor (PV), atopic dermatitis (AD), and psoriasis (PS). The Malassezia genus has repeatedly been revised and it now accommodates 14 species, all but one being lipid-dependent species. The traditional, phenotype-based...
متن کاملIsolation of Malassezia furfur from a cat.
During a survey of the occurrence of Malassezia species in the external ear canals of cats without otitis externa, Malassezia furfur was isolated. This is the first report of the isolation of M. furfur from cats.
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 25 10 شماره
صفحات -
تاریخ انتشار 1987